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            In rural, geographically dispersed school districts, access to high-quality face-to-face professional development (PD) is challenging. Our study developed and compared the effectiveness of an online PD for middle-school science teachers working in remote, rural areas of Kansas with an evidence-based traditional face-to-face PD. Fifteen rural middle-school science teacher participants were randomly selected to participate in the online or traditional PD, then taught the Toward High School Biology curriculum to their 504 middle-school students. Findings aligned with our hypothesis that online PD is as effective as traditional in improving student content knowledge. Teachers’ instructional practices in using Next Generation Science Standards improved, as did their use of student-centered instruction and making science relevant to the lives of their students.more » « less
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            Abstract Whitebark pine (WBP, Pinus albicaulis) is a white pine of subalpine regions in the Western contiguous United States and Canada. WBP has become critically threatened throughout a significant part of its natural range due to mortality from the introduced fungal pathogen white pine blister rust (WPBR, Cronartium ribicola) and additional threats from mountain pine beetle (Dendroctonus ponderosae), wildfire, and maladaptation due to changing climate. Vast acreages of WBP have suffered nearly complete mortality. Genomic technologies can contribute to a faster, more cost-effective approach to the traditional practices of identifying disease-resistant, climate-adapted seed sources for restoration. With deep-coverage Illumina short reads of haploid megagametophyte tissue and Oxford Nanopore long reads of diploid needle tissue, followed by a hybrid, multistep assembly approach, we produced a final assembly containing 27.6 Gb of sequence in 92,740 contigs (N50 537,007 bp) and 34,716 scaffolds (N50 2.0 Gb). Approximately 87.2% (24.0 Gb) of total sequence was placed on the 12 WBP chromosomes. Annotation yielded 25,362 protein-coding genes, and over 77% of the genome was characterized as repeats. WBP has demonstrated the greatest variation in resistance to WPBR among the North American white pines. Candidate genes for quantitative resistance include disease resistance genes known as nucleotide-binding leucine-rich repeat receptors (NLRs). A combination of protein domain alignments and direct genome scanning was employed to fully describe the 3 subclasses of NLRs. Our high-quality reference sequence and annotation provide a marked improvement in NLR identification compared to previous assessments that leveraged de novo-assembled transcriptomes.more » « less
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            The Bunker Hill Public Housing development is a historic public housing building, home to a large population of racial and ethnic minorities, that requires major redevelopment and repair to enhance the safety of its residents. The Boston Planning and Development Agency (BPDA) recently approved a $1.46 billion redevelopment for the property, a part of which is allocated to remove and replace ~250 mature trees around the public housing units. Removal of these trees would affect an already vulnerable population significantly more exposed to the effects of heat events, including heat-related stress, morbidity, and mortality, which will worsen with climate change in the coming years. While the BPDA proposal seeks to address the issue that the area already experiences 20% less cooling due to a lack of vegetation by replanting more trees, their estimated timescale of more than a decade for the canopy to just return to its current size is concerning. In order to mitigate the added heat stress caused by the tree removal, we propose the supplementary action of installing green roofs on buildings throughout the development. These green roofs would continue to provide cooling and beneficial community services even once the tree canopy has returned. These measures will serve as an appropriate stopgap measure until the canopy can return to size and expand as well as providing the community with the same co-benefits, such as air quality improvement, noise pollution reduction, community spaces, and locally grown food from community gardens, that more affluent parts of the city already experience. The installation of green roofs and supplemental vegetation will take only 0.25% of the entire redevelopment project budget and will have a large return in community wellness.more » « less
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